"Aoba Jitensha Ten" centers around Aoba Suzuki, a high school student who works part-time at a bicycle shop called "Ahobaka." The shop is run by the eccentric and somewhat lazy owner, Norifumi "Nori" Suzuki, who is also Aoba's older brother. The narrative explores the daily lives of the siblings and their interactions with customers, friends, and the broader cycling community. Aoba is passionate about bicycles and often finds herself repairing and customizing bikes for clients, showcasing her technical skills and deep knowledge of cycling mechanics.

The story is episodic, with each chapter typically focusing on a specific customer or situation. These vignettes highlight the diverse reasons people ride bicycles, ranging from practical commuting to competitive racing and personal hobbies. Through these interactions, Aoba and Nori help customers solve their problems, often using their expertise to repair or upgrade bicycles in ways that reflect the riders' needs and personalities. The series emphasizes the emotional connections people have with their bikes, portraying cycling as both a practical tool and a source of joy and freedom.

Aoba is depicted as a diligent and skilled mechanic, contrasting with Nori's laid-back and sometimes irresponsible demeanor. Despite their differences, the siblings share a strong bond and a mutual love for bicycles. Supporting characters include recurring customers, fellow cyclists, and members of the local community, each contributing to the series' exploration of cycling culture. The narrative occasionally delves into the technical aspects of bicycle maintenance and customization, appealing to readers with an interest in mechanics and engineering.

The tone of the series is lighthearted and heartwarming, with occasional moments of humor and introspection. It avoids heavy drama, instead focusing on the small, meaningful moments that arise from the characters' interactions and their shared passion for cycling. Through its episodic structure and focus on everyday life, "Aoba Jitensha Ten" offers a grounded and relatable portrayal of the joys and challenges of cycling, as well as the importance of community and craftsmanship.