On Earth, the eternal conflict between the Autobots and Decepticons continues, though in a much more lighthearted manner than usual. Rather than fighting for the fate of the universe, these giant robots often find themselves bickering over snacks, struggling with household chores, or simply trying to enjoy a day off without something going wrong. This is the world of Transformers Go! Go!, where the epic scale of the Generation 1 cartoon is shrunk down into small, comedic misadventures focused on the characters' daily lives and quirky personalities.

The story is driven by a large ensemble cast of recognizable characters. On the heroic side, the Autobot leader Optimus Prime often tries to maintain order, frequently assisted by the enthusiastic Bumblebee. The inventive but accident-prone Wheeljack is a constant source of chaos, as his new creations inevitably malfunction and cause trouble for everyone. Other Autobots like the cool-headed Jazz, the powerful Grimlock, and the booming Blaster also join in the fun. Their rivals, the Decepticons led by the ever-scheming Megatron, are just as likely to be found relaxing as they are plotting. Starscream chafes under constant praise for Megatron, while Laserbeak acts as a spy, gathering intelligence on the Autobots' daily activities.

The setting is a familiar, contemporary world where humans and Transformers coexist peacefully. The Autobots have a headquarters where they gather, but they are frequently seen enjoying downtown areas, engaging in snowball fights, and partaking in other leisurely activities. The tone is consistently soft and cheerful, with a round, cute art style designed for a younger audience. The series is structured as a gag-a-day comic, with each chapter presenting a self-contained, episodic story.

Several recurring narrative themes and arcs appear throughout the series. A significant portion of the comedy comes from the Autobots dealing with very human situations. One notable arc involves the robots discovering and becoming obsessed with human food, leading to an issue where they overeat and gain an excessive amount of weight. Another story follows the Autobots as they try to enjoy a peaceful stroll, only to become aware that they are characters in a manga being read by human children, leading to a very meta moment where they directly ask readers to buy the collected volume of their adventures. Seasonal stories are also common, with special chapters dedicated to celebrating Christmas and the New Year.

As the series progresses, the cast expands beyond the core Generation 1 cast. The Maximals and Predacons from the Beast Wars era eventually make their appearance, joining the Autobots and Decepticons in the lighthearted mayhem. The series also includes special issues, such as a Mini Encyclopedia that functions as a spotter's guide, providing profiles for the numerous characters and even teasing new ones yet to appear in the main comic.