"Cheer Danshi!! GO BREAKERS" centers on a group of male college students who form a cheerleading team, challenging societal norms and personal insecurities. The protagonist, Haruki Bandou, initially joins the team to support his childhood friend, Kazuma Hashimoto, who aspires to become a professional cheerleader. Haruki, a former judo athlete, struggles with self-doubt and a sense of purposelessness after quitting judo, but through cheerleading, he begins to rediscover his passion and determination.

The narrative explores the dynamics of the team as they navigate the challenges of building a competitive cheerleading squad from scratch. Each member brings unique struggles and motivations: Kazuma is driven by his dream to prove that men can excel in cheerleading, while others join for reasons ranging from personal growth to camaraderie. The team faces skepticism and ridicule from peers and institutions, as male cheerleading is often misunderstood or dismissed. Despite these obstacles, they work tirelessly to refine their skills, develop routines, and earn recognition.

The story delves into themes of perseverance, teamwork, and breaking gender stereotypes. The characters confront their fears and insecurities, learning to trust one another and embrace their individuality. As the team progresses, they compete in cheerleading competitions, facing off against more established squads and proving their worth through dedication and creativity. The series balances intense training sequences with moments of humor and heartfelt interactions, highlighting the bonds formed through shared struggles and triumphs.

"Cheer Danshi!! GO BREAKERS" also emphasizes the technical and artistic aspects of cheerleading, showcasing the physical demands and strategic planning required to succeed. The narrative underscores the importance of discipline, communication, and mutual support, both on and off the mat. Through their journey, the characters grow not only as athletes but also as individuals, challenging societal expectations and redefining what it means to pursue a passion. The series offers a compelling portrayal of underdogs striving to make their mark in an unconventional sport.