"Lady Go!!" is a sports manga centered around the world of women's professional wrestling. The narrative revolves around Asuka Tsuchiya, a young woman who aspires to become a professional wrestler after being inspired by the legendary wrestler Lady Z. Asuka joins the fictional wrestling promotion "Lady Go!!," where she trains alongside other aspiring wrestlers, each with their own motivations and backgrounds. The story explores the challenges and triumphs of Asuka and her peers as they navigate the physically demanding and emotionally intense world of professional wrestling.

The plot delves into the rigorous training regimens, the development of wrestling personas, and the interpersonal dynamics among the wrestlers. Asuka faces numerous obstacles, including self-doubt, rivalries, and the pressure to live up to the legacy of Lady Z. The series highlights the camaraderie and competition within the promotion, as well as the personal growth of the characters as they strive to achieve their dreams. Key themes include perseverance, teamwork, and the pursuit of excellence in a highly competitive field.

Main characters include Asuka Tsuchiya, the determined protagonist; Lady Z, the revered veteran wrestler who serves as a mentor figure; and a diverse cast of wrestlers, each with distinct personalities and wrestling styles. The interactions between these characters drive much of the narrative, with alliances and rivalries shaping the progression of the story. The manga provides a detailed look at the behind-the-scenes aspects of professional wrestling, from choreographing matches to managing public personas, offering a comprehensive portrayal of the sport.