"Disney Twisted-Wonderland The Comic: Episode of Heartslabyul" adapts the first story arc of the popular mobile game "Disney Twisted-Wonderland," focusing on the Heartslabyul dorm at Night Raven College. The narrative centers on Yuu, a mysterious protagonist who is transported to the magical world of Twisted Wonderland after being summoned by a magical mirror. Yuu, lacking any magical abilities, is placed under the care of the enigmatic headmage Crowley and becomes an honorary student at the academy.

The story primarily unfolds within Heartslabyul, one of the seven dorms at Night Raven College, each inspired by a Disney villain. Heartslabyul is modeled after the Queen of Hearts from "Alice in Wonderland," and its students are bound by strict rules and traditions. The dorm is led by Riddle Rosehearts, a perfectionist and authoritarian housewarden who enforces the rules with unwavering rigidity. Riddle's obsession with order stems from his upbringing and his belief that rules are essential for maintaining harmony.

Yuu's arrival coincides with growing tensions within Heartslabyul, particularly between Riddle and Ace Trappola, a rebellious first-year student who frequently challenges the dorm's strict regulations. Ace's defiance escalates when he eats a tart meant for an unbirthday party, a serious violation of Heartslabyul's traditions. This act triggers a confrontation with Riddle, who uses his unique magic, "Off With Your Head," to punish Ace by sealing his magic. The conflict deepens as Ace, along with his friend Deuce Spade and Yuu, seeks to challenge Riddle's authority and the oppressive nature of the dorm's rules.

As the story progresses, it becomes clear that Riddle's rigid adherence to rules is rooted in his traumatic childhood, where his overbearing mother imposed strict control over his life. This backstory humanizes Riddle and provides context for his behavior. The climax of the arc involves a dramatic showdown between Riddle and the protagonists, culminating in Riddle's realization that his extreme methods have alienated those around him. Through this confrontation, Riddle begins to question his beliefs and opens the door to potential growth and change.

The narrative explores themes of authority, individuality, and the consequences of rigidly imposed rules. It also introduces key characters such as Trey Clover, Riddle's childhood friend and vice-housewarden, who serves as a moderating influence, and Cater Diamond, a charismatic upperclassman who navigates the dorm's social dynamics with ease. The story sets the stage for further exploration of Night Raven College and its other dorms, each with its own unique challenges and characters.