"Teikō Penguin" centers on a group of penguins living in a zoo, each with distinct personalities and roles within their community. The narrative explores their daily lives, interactions, and the challenges they face in maintaining harmony within their group. The protagonist, a young and ambitious penguin named Peso, aspires to become the leader of the colony, a position currently held by the wise but aging leader, Taka. Peso's journey is marked by his efforts to prove his worth, often leading to humorous and sometimes perilous situations.

The plot unfolds through a series of episodic adventures, where the penguins encounter various obstacles, both internal and external. These include rivalries with other zoo animals, human visitors, and environmental changes affecting their habitat. Peso's determination and resourcefulness are frequently tested, and his growth is a central theme. Alongside Peso, key characters include Miko, a clever and supportive friend who often aids Peso in his endeavors, and Goro, a rival penguin who challenges Peso's leadership aspirations.

The series balances lighthearted moments with deeper themes of responsibility, teamwork, and perseverance. The penguins' interactions with humans, particularly the zookeepers, add another layer to the story, highlighting the dynamics between animals and their caretakers. The narrative also touches on environmental issues, subtly addressing the impact of human activity on wildlife.

"Teikō Penguin" is structured to appeal to a broad audience, combining humor, adventure, and emotional depth. The episodic nature allows for standalone stories while maintaining an overarching progression of character development and relationships. The series effectively captures the essence of life within a zoo, presenting a unique perspective through the eyes of its penguin protagonists.