Natsuko Aki in Re: Cutie Honey is a police detective working for the National Police Public Safety Agency, serving as a lead investigator for the National Police Security Task Force Branch 9. Unlike earlier versions of the character who were typically high school students, this iteration is depicted as a competent and formidable professional. She is cold, controlling, and an absolute perfectionist, with a stern demeanor that reflects her commitment to order and discipline. Despite her exceptional skills as a police officer, she has a tendency to get in over her head when pursuing cases.

Her primary role in the story involves investigating the mysterious vigilante known as Cutie Honey. During one investigation, she discovers Honey's identity and attempts to arrest her for her revealing costume and interference with police work, though she ultimately loses track of her when Honey receives help from the reporter Seiji Hayami. Natsuko’s motivations are driven by a strong sense of duty and a desire to maintain public order, though her rigid approach sometimes puts her at odds with the more unorthodox methods of those around her.

Natsuko lives alone in a small apartment and frequently wears black clothing, often a business suit, with her black hair kept in a ponytail. She hides her appearance behind non-prescription glasses. Outside of her professional life, she has a passion for Japanese sake and enjoys singing karaoke when intoxicated. She is also an expert marksman, a skill she employs in her police work. Her development as a character centers on her interactions with the more chaotic world of Cutie Honey, challenging her controlled nature and forcing her to adapt. Key relationships include her adversarial yet intertwined dynamic with Cutie Honey and her association with Seiji Hayami, who aids Honey in evading capture.