Natalie Van Sistine is an American voice actor, audio engineer, and script writer known for her extensive work in English-language dubs of anime, as well as her roles in video games and audio dramas. Her career in voice acting began after an initial pursuit of music was altered due to health issues, leading her to discover online communities dedicated to audio drama and collaborative projects around 2008. She transitioned from this hobby into professional work, initially supporting her acting with a background in audio engineering, a field she studied formally and which led her to relocate to the Dallas-Fort Worth area, a hub for anime production. A pivotal moment in her early career was being cast by fellow actor and director Amber Lee Connors in her first anime role, Rosa in Rio: Rainbow Gate, a connection that helped establish her in the industry.

Van Sistine's most prominent and career-defining role is as Yor Forger in the immensely popular spy comedy series Spy×Family. She prepared for the audition by reading the original manga, which helped her connect with the character's duality as both a nervous, anxious wife and a cold, professional assassin. The role brought her widespread recognition, leading to invitations to conventions worldwide, a theatrical film, and various promotional partnerships. She has described voicing Yor as a life-changing experience that redirected the trajectory of her career.

Beyond Spy×Family, Van Sistine has voiced a wide array of notable anime characters. Her filmography includes Miorine Rembran in Mobile Suit Gundam: The Witch from Mercury, a character she has cited as a personal favorite for her complex and spirited personality. She also voices Star and Stripe in My Hero Academia, the Fox Devil in Chainsaw Man, Selene in Fairy Tail: 100 Years Quest, Arnheid in Vinland Saga, and Natsuko Aki in Re: Cutie Honey. In addition to these, her extensive list of roles includes those mentioned in the query: Solow in Smile of the Arsnotoria the Animation, Lilith in The Maid I Hired Recently Is Mysterious, Reona Kisaragi in Full Dive, Sofia in Arte, Irene Adler (also known as James Bonde) in Moriarty the Patriot, Ren Hazuki in Love Live! Superstar!!, Suzune Horikita in Classroom of the Elite II, and Akane Matsui in Fire in His Fingertips 2 -My Boyfriend is a Fireman-.

Her versatility extends into video games, where she has provided voices for over two hundred titles. She is well-known as Serval Landau in Honkai: Star Rail, a role she particularly enjoys and feels a personal connection to. Other significant game credits include Bianca North in Death Stranding 2, Dark Ranger Velonara in World of Warcraft, and roles in Hearthstone, Fire Emblem Heroes, Goddess of Victory: Nikke, and Final Fantasy VII: The First Soldier.

In addition to her on-screen work, Van Sistine is a multifaceted professional who works behind the scenes in anime production. Leveraging her training as an audio engineer, she has served as an ADR engineer, lead engineer, and script writer for numerous series. Her engineering credits include Kageki Shojo!!, Dragon Goes House-Hunting, and Adachi and Shimamura, while her script adaptation work includes Mobile Suit Gundam: The Witch from Mercury, The Prince of Tennis, and Fist of the North Star. She has noted that her engineering experience provides her with a comprehensive understanding of how an anime dub is assembled, which in turn benefits her work as an actor. Outside of anime and gaming, she has also performed as Nesta in the Graphic Audio production of the popular book series A Court of Thorns and Roses.