Tamako Nekoya is a supporting character appearing across official media. She is one of the Nekoya twins, alongside her sister Suzuko. Both sisters share ochre hair and brown eyes, with their cat-related surname reflecting the series' animal motif naming conventions.

Tamako embodies vanity and self-centered tendencies, often prioritizing individual strutting over team coordination during cheer activities. She displays playful cat-like mannerisms, including teasing expressions and taunting behavior directed at others. Tamako and Suzuko previously participated in cheerleading as Hizume Arima's teammates before the main storyline. Their interactions with Hizume carry antagonistic undertones; they openly express satisfaction that her absence from their former team granted them greater prominence.

The twins reappear during a training camp arc, encountering the main characters away from their hometown. During this interaction, they reiterate their dismissive attitude toward Hizume's current cheerleading pursuits. Their presence primarily serves to highlight aspects of Hizume's backstory and catalyzes defensive reactions from characters like Kana Ushiku, who admires Hizume.