Peter Parker works as a scientist at Stark Industries, aiding Dr. Akatsuki in developing DISK technology. When Loki attacks the project unveiling, he evades capture while other heroes are sealed into DISKs. This allows him to retrieve the Avengers' DISKs and distribute them to biocode-equipped children, enabling temporary hero releases during later conflicts.

His scientific expertise repeatedly supports the team, including collaborations with Akira Akatsuki and Iron Man to halt the Lizard’s New York rampage. He attempts to recruit Chris Taylor for the DISK initiative, though failing initially. During this effort, foes like Abomination capture him, resulting in brief imprisonment with other heroes on a villains’ frigate. Jessica Shannon and Edward Grant later free him with the Wasp’s help.

After the Superhero Registration Act passes, he resigns from the Avengers to continue heroics locally in New York. He visits the team’s base to deliver a video explaining this choice, discreetly preserving his secret identity when acknowledged for Spider-Man’s efforts.

A pivotal moment unfolds when he discovers the Venom symbiote while inspecting a wrecked S.H.I.E.L.D. truck. The alien bonds with him, forming a black suit that heightens aggression and triggers violent acts against criminals, tarnishing his reputation. The Avengers intervene upon noticing this shift, uncovering the symbiote’s sonic vulnerability. They employ a giant bell to sever it from Peter. After the symbiote possesses the Hulk, he aids in its ultimate defeat. Urged by Captain America, he publicly apologizes for his symbiote-influenced behavior. Footage of his heroism, sent by the children, helps redeem his standing.

He joins major battles, combating Mindless Ones during Dormammu’s New York invasion and aiding in Loki’s final defeat by overwhelming his army.

His gear features self-engineered web-shooters using specialized "web fluid" for mobility and combat, showcasing his scientific ingenuity.