Ayano Kondou is Kenji Kondou's mother and serves on the Alvis operational staff at Tatsumiya Island, primarily managing the critical Brunhilde System defensive component. She expresses visible relief upon Kenji's safe return after a Festum attack, aware of the inherent dangers. Ayano clarifies that the computer equipment in their home is hers, confirming her technical role. During strategic meetings on island relocation, she reports the Brunhilde System's standby status, noting its previous management by Kouzou Minashiro. Though initially reluctant, she accepts command of the system when tasked by Fumihiko Makabe. Ayano becomes visibly distressed upon learning Kenji is assigned as a Fafner pilot, highlighting her protective maternal instincts conflicting with military necessity. Her technical expertise proves crucial when the Brunhilde System activates unexpectedly during an urgent Festum alert, enabling defensive preparations despite challenges.

Later, Ayano appears in commemorative contexts. Photographs show her alongside Seiichirou Kaname at Sakura Kaname and Kenji Kondou's wedding, indicating her presence in the community's memory. She is later depicted among deceased individuals who manifest before Kenji and Sui Kaburagi, confirming her eventual passing. Kenji revisits photos of her and other departed loved ones while discussing threats with Soushi Minashiro, underscoring her lasting emotional significance to her son.