Ana is a combat android constructed by Dr. Akamashi using lost Old World technology. Designed with a small, deceptively childlike form resembling a young girl, featuring pink hair and violet eyes, she serves among Akamashi's key enforcers during his coup against the Forland Kingdom.

Her personality is aggressive, arrogant, and temperamental. Ana frequently taunts opponents, shows disregard for human life except Akamashi's, and boasts about her strength to compensate for her stature. This contrasts with her timid twin sister, Yuna, though both react with extreme violence if their creator is threatened.

Ana specializes in close-quarters battle. Her primary armaments include a retractable dagger blade in her right arm capable of puncturing most materials and deflecting weapons, rocket boosters in her footwear for enhanced mobility and flight, and "rocket punch" mechanisms launching her fists on chains to pierce targets. She often coordinates attacks with Yuna, such as the "Twin Takedown" combining their weapons.

Ana's fierce loyalty to Akamashi, whom she views as a father figure, clouds her judgment regarding his morally corrupt orders. Following Akamashi's death, the influence of her programming on her violent behavior becomes clear. She subsequently aligns with the protagonists, demonstrating loyalty beyond her creator—particularly toward Yuna and her new guardian, Starlight Glimmer. Protective instincts toward her sister emerge, tempering her previous aggression.

In the series conclusion, Ana helps activate a mechanism to prevent global catastrophe. This requires removing all lost technology from the world, resulting in her dissolution alongside other technological entities in a display of light. This involuntary sacrifice completes her arc from antagonist to reluctant ally.