Nino Nakano, the second oldest quintuplet, initially wears long hair with twin butterfly-shaped ribbons, later cutting it short as a symbol of change. Her fashionable style includes careful attention to accessories and nail care, though she avoids ear piercings. Academically ranking lower among her sisters, English is her strongest subject. Poor eyesight requires contact lenses, sometimes causing difficulty recognizing people. Following her mother's death in childhood, Nino shouldered significant familial responsibility. She became a caretaker, developing cooking skills to support her sisters, especially through making pancakes—a dish linked to her mother's memory. This culinary ability reflects her underlying care and protectiveness beneath a rebellious exterior. Early conflicts with her adoptive father, Maruo, stemmed from his restrictions on activities reminding them of their mother, strengthening Nino's resolve to preserve family bonds. Initially, Nino displays intense hostility towards Futaro Uesugi, seeing him as an outsider disrupting the sisters' dynamic. She openly rejects his tutoring and even drugs him twice with sleeping pills. Her protectiveness manifests as resistance to change, fearing separation as her sisters pursue individual paths, rooted in anxiety about losing familial unity. This leads to clashes with Futaro and her sisters, especially Miku. Her character development centers on accepting change and confronting emotions. Cutting her hair precedes acknowledging her feelings for Futaro; she becomes the first sister to confess to him directly and unabashedly. This marks a shift from antagonism to open, proactive affection, including bold, flirtatious advances like attempting intimacy in a hot spring. Her arc emphasizes embracing vulnerability and supporting her sisters' autonomy despite rivalry. Later, Nino opens a bakery with Miku, fulfilling her culinary dream. During Futaro and Yotsuba's honeymoon in Hawaii, she encounters a girl seeking romance advice. Despite limited English, she collaborates with her sisters to help. This reinforces her growth in relying on teamwork while asserting independence, like declining Futaro's help to handle workplace challenges alone, such as serving foreign customers despite language insecurities. Her personality blends assertiveness with deep loyalty. Socially adept yet blunt, often using sharp language, she also shows empathy, consoling Futaro about his past or advising Yotsuba on honesty. Romantic idealism contrasts with pragmatic resilience, seen in her rivalry with Yotsuba after Futaro's choice; she accepts the outcome but vows to compete if opportunities arise, demonstrating unwavering determination. She is attracted to blonde individuals, uses a rabbit-themed phone cover, fears needles, and prefers black tea. Purple rose petals symbolizing "love at first sight" feature on a manga volume cover.