Last Order, designated Misaka 20001, is the ultimate clone of the Level 6 Shift Project, operating as the Misaka Network’s central command hub and fail-safe administrator. Her physical growth was deliberately frozen in a childlike state resembling a 10-year-old Mikoto Misaka, distinguished by a signature ahoge, pink cheek marks, and eyes mirroring Mikoto’s original hue—unlike her clone siblings. Initially draped in tattered cloth, she later adopts a vibrant blue polka-dot dress, white coat, and sandals. Emotionally vivid compared to her subdued counterparts, Last Order radiates playful curiosity, communicates in third-person speech peppered with dual "Misaka" references, and forges deep bonds with Accelerator, Yomikawa Aiho, Yoshikawa Kikyou, and Misaka 10032. Though harboring the collective memories of all clones, including those slain by Accelerator, she discerns his reluctant participation in the experiments, absolving him of blame. Her sharp intuition deciphers Accelerator’s hidden intentions and Touma Kamijou’s pivotal role in halting the project. Despite peril, she selflessly intervenes to rescue Fräulein Kreutune, showcasing unwavering empathy. Prematurely awakened after the project’s termination, she debuts physically in the Three Stories Arc, weakened and collapsing before becoming a pawn in Kihara Amata’s virus plot during the Academy City Invasion. Accelerator’s sacrificial intervention eradicates the malware, cementing their protective bond and catalyzing his redemption. Relocating with Yomikawa and Yoshikawa, she balances mundane life with her network duties. Her agency escalates in later arcs: she collaborates with Uiharu Kazari to track Accelerator during the Battle Royale Arc, leveraging electromagnetic sensing to navigate urban chaos. Targeted by Disciplinary Action (DA) in the Necromancer Arc, she triggers Accelerator’s defensive fury. As the network core, she thwarts viruses, manipulates data streams, and remains a coveted asset for adversaries. Her Level 3 Radio Noise abilities exceed standard clones’ electrical output, paired with technokinetic hacking. Network access lets her fabricate phenomena, sever Accelerator’s network-dependent powers, and retrieve shared clone memories. Despite her youthful guise, her tactical influence anchors scientific and supernatural clashes. Evolving from fragility to proactive force, Last Order reshapes Accelerator’s identity, challenging his villainous self-image. In the Tree Diagram Remnant Arc, she urges him to protect the clones, pivotal to his moral rebirth. Her capacity to forgive aggressors like Kreutune and prioritize individual lives reveals maturity beyond her form, positioning her as a ethical keystone amid Academy City’s moral ambiguities.