Last Order stands as the ultimate clone created for the Level 6 Shift Project, specifically excluded from the termination order targeting 20,000 other clones. She operates as the command center and administrator of the Misaka Network, granting her authority over all sister clones. Researchers built in a fail-safe mechanism allowing external control of the network through specific brainwave signals directed at her, requiring her perpetual childlike form for easier manipulation. Her name derives from the scenario where she would command the clones to shut down. Physically, Last Order appears as a 10-year-old Mikoto Misaka, complete with a reactive ahoge strand. She first appeared in ragged makeshift clothing but later adopted proper attire: a blue spotted dress, white coat, and sandals. Unlike her sisters, her eye color matches the original Mikoto, and she shares Mikoto's sensitivity about her chest size. Personality-wise, Last Order exhibits significantly greater emotional expressiveness than her clone sisters. She communicates uniquely in third person, using "Misaka" twice in her speech. Her demeanor is playful and curious, exemplified by actions like snatching Misaka 10032's goggles to play or initiating games of tag. Despite these childish tendencies, she demonstrates perceptiveness, notably theorizing Accelerator never truly desired participation in the Level 6 Shift Project, thereby absolving him of blame for his actions. She deeply admires Kamijou Touma for halting the experiments and protecting her during critical moments. She also exhibits caring traits, risking her life to save Fräulein Kreutune despite imminent danger. Her chronological development began during the Three Stories Arc on August 31, when she approached Accelerator seeking help to locate researchers for a return to her incubator. After collapsing from physical weakness, Amai Ao abducted her and activated an internal virus. Accelerator intervened, deleting the virus at great personal risk, facilitating her rescue and hospitalization under Misaka 10032's care. She subsequently came under the guardianship of Yomikawa Aiho and Yoshikawa Kikyou. In the Tree Diagram Remnant Arc, Last Order communicated with clones about the potential experiment restart due to the remnant's existence, deliberately hiding her connection to Accelerator. She later persuaded Accelerator to destroy the remnant, prompting his vow to become "the strongest" for her sake. Their dynamic features comedic exchanges, including her disabling his speech via the network and his mock-strangulation attempts. During the Academy City Invasion Arc, Kihara Amata captured Last Order and implanted a virus corrupting the Misaka Network, exploiting Kazakiri Hyouka for Aleister Crowley's plans. Accelerator's actions and Index's curative intervention secured her rescue, though the organization Group later leveraged her safety to recruit Accelerator. After recovery at Heaven Canceller's hospital, she was released during the Battle Royale Arc. While searching for Accelerator, she encountered Uiharu Kazari of Judgment after abandoning a taxi. Uiharu gifted her a hibiscus flower for encouragement. Last Order sensed Accelerator's proximity but failed to locate him before he departed for School District 23. Her pivotal role as network administrator surfaces during crises. When Kakine Teitoku stabbed Aiho Yomikawa, triggering Accelerator's berserk state manifested as black wings, Last Order intervened to pacify him, demonstrating her unique influence over his emotional stability.