Last Order, designated Misaka clone 20001, serves as the command center and administrator of the Misaka Network. She emerged as the final clone in the Level 6 Shift Project, intentionally halted in development to resemble a 10-year-old version of Mikoto Misaka for easier researcher control. Unlike the 20,000 clones slated for elimination by Accelerator, she avoided that fate. Her appearance mirrors the original Mikoto's eyes and features a distinctive reactive ahoge. Initially clad only in a ragged blanket, she later adopted a blue spotted dress, white coat, and sandals. Functioning as the network's fail-safe, Last Order allows researchers to transmit brainwave signals for external control if clones grow unmanageable. Her name derives from her programmed capacity to issue a network-wide shutdown command. She retains all clone memories via the network, witnessing events like Accelerator's involvement in the Level 6 Shift Project firsthand. Personality-wise, Last Order exhibits unique emotions among clones—playful, curious, and perceptive. She speaks in third person, repeating "Misaka" twice per sentence. Her behavior includes stealing Misaka 10032's goggles or initiating tag games. She demonstrates care through actions like risking her life to save Fräulein Kreutune. Her perceptiveness lets her theorize about Accelerator's reluctance in the experiments, leading her to absolve him of blame. She admires Kamijou Touma for halting the experiments and protecting her. Her chronology begins on August 31, when she approaches Accelerator for help contacting researchers after premature incubation release. He ignores her initially but relents after seeing her vulnerability, letting her stay at his apartment. At a family restaurant, she collapses due to physiological instability and is abducted by researcher Amai Ao, who implants a network-corrupting virus set to trigger indiscriminate human attacks at midnight on September 1. Accelerator deletes the virus but sustains a severe head injury during the confrontation. Recovering under medical care with Misaka 10032 as her attendant, Last Order later moves in with Accelerator and guardians Yomikawa Aiho and Yoshikawa Kikyou. During the Academy City Invasion arc, Kihara Amata captures her to corrupt the network again, though Accelerator and Index rescue her. The paramilitary Group subsequently leverages her safety to recruit Accelerator. In later events, hospitalized for recovery, she leaves prematurely to search for Accelerator. She encounters Uiharu Kazari of Judgment, who briefly assists her. Last Order demonstrates an ability to sense Accelerator's approximate location through the network, evading Anti-Skill and resting at public venues like cafés. She reappears during conflicts involving Accelerator, such as his battle against Kakine Teitoku, where her safety indirectly influences his actions. Her relationships define her development. She shares a familial bond with Accelerator, who evolves into her protector despite initial hostility. Close ties with Yomikawa Aiho, Yoshikawa Kikyou, and Misaka 10032 reflect her integration into non-experimental life. Her network connection persists, enabling coordination with other clones during crises like the Tree Diagram Remnant incident, where she secretly requests Accelerator's help to destroy the device.