"Ai Mai Mi" is a comedic manga series centered around the absurd and surreal adventures of its main characters: Ai, Mai, and Mi, who are members of their school's manga club. The narrative is episodic, with each chapter presenting a self-contained story that often blends slapstick humor, meta-commentary, and bizarre situations. The trio frequently engages in exaggerated, over-the-top antics, ranging from mundane school activities to fantastical scenarios, such as battling aliens, time travel, or confronting supernatural entities. Despite the chaotic nature of the plot, the series maintains a lighthearted tone, with the characters' personalities driving much of the humor.

Ai is the most level-headed of the group, often serving as the straight man to the others' eccentric behavior. Mai is impulsive and energetic, frequently instigating the group's misadventures, while Mi is quiet and reserved, often providing deadpan reactions that contrast with the absurdity around her. The manga club's advisor, Sensei, adds another layer of comedy, often being drawn into the girls' antics or displaying her own quirks. The series frequently breaks the fourth wall, with characters acknowledging their roles in a manga and commenting on the narrative structure or artistic choices.

The storyline lacks a continuous plot arc, instead focusing on rapid-fire gags and surreal humor. Themes of friendship and creativity are subtly woven into the narrative, as the characters' shared passion for manga often serves as the catalyst for their adventures. The series thrives on its unpredictability, with each chapter offering a new, often nonsensical scenario that keeps the tone fresh and engaging. Despite its comedic focus, "Ai Mai Mi" occasionally touches on moments of introspection or emotional resonance, though these are quickly overshadowed by the next wave of absurdity. The manga's strength lies in its ability to balance its chaotic humor with a sense of charm and relatability, making it a distinctive entry in the comedy genre.