Mayu Isshiki is a Japanese voice actress affiliated with the agency Arts Vision. She was born on December 24, 1980, in Kyoto Prefecture. Her hobbies include singing jazz and bossa nova music.

Isshiki is known for her work in anime and video games. In the Pretty Cure franchise, she has voiced several characters, notably Azukina, the fairy and fiancée of Tarte, in the 2009 television series Fresh Pretty Cure!. She also provided voices for the Pretty Cure film Fresh Pretty Cure! The Toy Kingdom is Full of Secrets!? and had earlier, uncredited roles in Yes! Pretty Cure 5 and its sequel Yes! Pretty Cure 5 GoGo!. Her other anime voice roles include parts in the series Major (fourth season), Card Fighters - Shot, and Kenji Miyazawa's Restaurant. She also voiced the character Chihiro in the anime adaptation of the visual novel I Like You, Suzuki-kun!!.

In video games, Isshiki has a prominent role as the voice of the Zora princess Mipha in the acclaimed The Legend of Zelda: Breath of the Wild, a role she reprised for Hyrule Warriors: Age of Calamity. She later returned to the franchise to voice the former Sage of Water in the sequel, Tears of the Kingdom. In the 2025 game Hyrule Warriors: Age of Imprisonment, she is credited as the Japanese voice for the character Qia.