"Fresh Pretty Cure" is a magical girl anime that centers around Love Momozono, a cheerful and energetic middle school student who becomes one of the guardians tasked with protecting the world from evil forces. The story begins when Love encounters a mysterious fairy named Chiffon, who is being pursued by agents of the Labyrinth, a malevolent organization seeking to conquer the land of sweets, the Kingdom of Sweets. To protect Chiffon and the kingdom, Love transforms into Cure Peach, a Pretty Cure warrior, gaining the power to fight against the Labyrinth's minions.

As the series progresses, Love is joined by three other girls who also become Pretty Cure warriors: Miki Aono, who transforms into Cure Berry, and Inori Yamabuki, who becomes Cure Pine. Together, they form a team dedicated to defending the Kingdom of Sweets and their own world from the Labyrinth's schemes. Each character brings unique traits to the group: Love is optimistic and determined, Miki is confident and stylish, and Inori is shy but kind-hearted. Their teamwork and growing friendship are central to the narrative, as they learn to balance their personal lives with their responsibilities as Pretty Cure warriors.

The Labyrinth, led by the enigmatic Moebius, seeks to spread despair and negativity by targeting people's hearts and turning them into "Nakewameke," creatures born from negative emotions. The Pretty Cure team must confront these threats while also dealing with the personal challenges of adolescence, such as school, family, and relationships. Over time, they uncover the truth about the Labyrinth's motives and the connection between their world and the Kingdom of Sweets.

The series features a mix of episodic battles and overarching plotlines, with the Pretty Cure team facing increasingly powerful enemies as they grow stronger and more united. Key antagonists include members of the Labyrinth's elite, such as Westar, Soular, and Eas, each with their own motivations and conflicts. Eas, in particular, undergoes significant character development, transitioning from a loyal servant of the Labyrinth to a complex figure torn between duty and her own desires.

"Fresh Pretty Cure" balances action, humor, and emotional moments, exploring themes of friendship, perseverance, and the importance of hope. The narrative builds toward a climactic confrontation with the Labyrinth, where the Pretty Cure team must harness their full power to protect both worlds and restore balance. The series concludes with a resolution that ties together the characters' growth and the overarching conflict, leaving a lasting impact on their lives and the worlds they have fought to save.