"On Air Dekinai!" centers on the chaotic and often humorous behind-the-scenes world of a struggling late-night radio show. The story unfolds in a small, underfunded radio station where a group of eccentric and mismatched staff members work tirelessly to keep their program on air. The main characters include the show's inexperienced but passionate producer, a cynical and sharp-tongued director, a scatterbrained sound engineer, and a quirky cast of radio personalities who each bring their own unique quirks and challenges to the table.

The narrative explores the daily struggles of the team as they navigate technical malfunctions, tight deadlines, and interpersonal conflicts, all while trying to maintain their audience's interest. The show's survival is constantly at risk due to low ratings, budget cuts, and the station's lack of resources. Despite these obstacles, the team's camaraderie and determination drive them to come up with creative solutions, often leading to absurd and comedic situations.

Key plot points include the introduction of a new, inexperienced host who must quickly adapt to the chaotic environment, a rival radio show that threatens to overshadow their program, and a series of unexpected events that test the team's resilience. The story balances humor with moments of genuine tension and heartfelt interactions, highlighting the challenges of working in a high-pressure, low-reward industry.

The characters' growth is a central focus, as they learn to overcome their individual flaws and work together to keep the show alive. Their relationships evolve through shared struggles, leading to moments of both conflict and solidarity. The series provides a detailed and often exaggerated look at the realities of radio production, blending workplace comedy with a touch of drama to create an engaging and relatable narrative.