"Nee, Chanto Shiyō yo!" is a short-form anime series that revolves around the daily lives of two sisters, Nee and Chanto, as they navigate their contrasting personalities and humorous interactions. Nee, the older sister, is responsible and diligent, often taking charge of household tasks and ensuring everything runs smoothly. In contrast, Chanto, the younger sister, is carefree and somewhat lazy, frequently relying on Nee to handle responsibilities. The series explores their comedic dynamic as they tackle mundane yet relatable situations, such as chores, schoolwork, and sibling rivalry.

The narrative is episodic, with each episode focusing on a specific scenario that highlights the sisters' differing approaches to life. Nee's perfectionism and Chanto's laid-back attitude often lead to amusing conflicts, but their bond as sisters remains central to the story. Despite their differences, they care deeply for each other, and their interactions are filled with warmth and humor. The series captures the essence of sibling relationships, blending lighthearted comedy with moments of genuine connection.

Supporting characters, such as friends and classmates, occasionally appear to add variety to the storylines, but the focus remains primarily on Nee and Chanto. The simplicity of the plot and the relatable nature of the characters make the series accessible and enjoyable for a wide audience. Through its short episodes, "Nee, Chanto Shiyō yo!" effectively portrays the ups and downs of sibling life, offering a charming and entertaining glimpse into the everyday adventures of two sisters.