Nene Sakurada appears as an ancestor in "Crayon Shin-chan: Arashi o Yobu Appare! Sengoku Daikassen". This historical figure, named Nene (ねね) and written with different characters than her modern descendant Nene (ネネ), lives in Kasuga fortress during the Sengoku period. Visually, she closely resembles her present-day counterpart, sharing her distinctive physical appearance and likely aspects of her general demeanor.

A key distinction emerges in their fears: while modern Nene channels frustration by hitting a stuffed bunny rabbit, her Sengoku-era ancestor harbors an extreme fear of actual rabbits.

Within the fortress children's group, this Nene interacts with the ancestors of Shin-chan's friends—Omasa (Masao), Kazuma (Kazama), and Bōshichi (Bo). Their group dynamic reflects familiar social patterns; Omasa leads but displays cowardice mirroring Masao when facing danger, like bandit attacks.

She operates within the film's historical narrative, encountering the time-displaced Shinnosuke Nohara during his past adventures. Her presence, alongside the other ancestral children, provides a recognizable link to the main series' ensemble within this unique setting.