"Ro-Kyu-Bu! Yonkoma" is a four-panel manga spin-off of the "Ro-Kyu-Bu!" series, which originally began as a light novel. The story revolves around Subaru Hasegawa, a high school student who is reluctantly recruited to coach a girls' elementary school basketball team after his own team is suspended due to a scandal. The team, consisting of five girls—Tomoka Minato, Maho Misawa, Saki Nagatsuka, Airi Kashii, and Hinata Hakamada—struggles with their lack of experience and coordination. Subaru, despite his initial reluctance, gradually becomes invested in helping the girls improve their skills and bond as a team.

The narrative focuses on the daily lives and interactions of the characters, blending lighthearted comedy with moments of growth and camaraderie. Each chapter typically highlights the girls' attempts to learn basketball, their school life, and their relationships with Subaru, who often finds himself in awkward but humorous situations due to the age gap and the girls' playful personalities. The manga emphasizes the comedic aspects of the original series, with a particular focus on the girls' antics and their efforts to balance basketball practice with their everyday lives.

The characters are distinct in their personalities and roles within the team. Tomoka, the team captain, is energetic and determined but often struggles with self-doubt. Maho is the most skilled player but has a sharp tongue and a competitive streak. Saki is shy and reserved, often serving as the voice of reason, while Airi is cheerful and optimistic, providing comic relief. Hinata, the youngest, is innocent and enthusiastic, often bringing a sense of innocence to the group dynamics. Subaru, as the coach, acts as a stabilizing force, guiding the girls while navigating the challenges of coaching a team of inexperienced players.

The manga maintains a light and episodic structure, with each chapter presenting self-contained scenarios that contribute to the overall development of the characters and their relationships. The humor often arises from the contrast between the girls' youthful exuberance and Subaru's more reserved demeanor, as well as their frequent misunderstandings and playful teasing. While basketball serves as a central theme, the focus is more on the characters' interactions and growth rather than intense sports action, making it accessible to readers who may not be familiar with the sport.

Overall, "Ro-Kyu-Bu! Yonkoma" offers a comedic and heartwarming take on the original series, emphasizing the bonds between the characters and their shared journey of improvement, both on and off the court.