"Mitchiri Wanko! Anima~tion" is a short-form anime series that revolves around the daily lives of a group of anthropomorphic dogs and their interactions with each other and their human owner. The dogs, each with distinct personalities and quirks, live together in a household where their playful and often chaotic antics drive the narrative. The series is episodic, with each episode focusing on humorous and lighthearted scenarios that highlight the dogs' unique traits and their relationships.

The main characters include Wanko, a cheerful and energetic dog who often takes the lead in the group's adventures; Chibi, a small and timid dog who is easily frightened but deeply loyal; and Kuro, a laid-back and somewhat aloof dog who often provides a contrast to the others' hyperactivity. Other members of the group include Shiro, a calm and intelligent dog, and Pochi, a mischievous and playful pup. Their human owner, referred to as "Master," is a caring but often exasperated figure who tries to manage the chaos caused by the dogs.

The plot is driven by the dogs' everyday activities, such as playing games, exploring their surroundings, and occasionally causing trouble for their owner. The series emphasizes humor and charm, with the dogs' exaggerated reactions and slapstick comedy serving as the primary source of entertainment. Despite their antics, the dogs share a strong bond with each other and their owner, often coming together to solve problems or support one another in times of need.

The tone of the series is consistently light and family-friendly, with no overarching conflict or dramatic tension. Instead, the focus remains on the dogs' endearing personalities and their ability to find joy in the simplest of situations. The episodic structure allows for a variety of comedic scenarios, ensuring that each episode feels fresh and engaging while maintaining the core themes of friendship and fun.