"Wake up!! TAMALA" centers on Tamala, a young girl who awakens in a mysterious world after a sudden accident. The narrative unfolds in a surreal, dreamlike setting where reality and fantasy blur. Tamala discovers she possesses unique abilities tied to the enigmatic "Dream Fragments," scattered remnants of memories and emotions that hold the key to understanding her past and the world around her. As she navigates this strange realm, she encounters a diverse cast of characters, each with their own connections to the Dream Fragments and their own motivations for seeking them.

The plot is driven by Tamala's quest to recover the Dream Fragments, which are guarded by powerful entities known as "Dreamkeepers." These guardians test her resolve and challenge her understanding of herself and her purpose. Along the way, Tamala forms alliances with other seekers, including Kaito, a pragmatic and resourceful companion, and Lumi, a mysterious girl with her own hidden agenda. Their journey is fraught with tension, as rival factions and shadowy figures also vie for control of the Dream Fragments, believing them to hold the power to reshape reality.

Themes of identity, memory, and the nature of existence are woven throughout the story, as Tamala grapples with fragmented recollections of her life before the accident. The narrative explores the interplay between dreams and reality, questioning whether the world she inhabits is a construct of her mind or an independent, tangible realm. As Tamala uncovers more Dream Fragments, she begins to piece together not only her own history but also the larger mysteries of the world and its origins.

The series balances episodic adventures with an overarching plot, gradually revealing the true nature of the Dream Fragments and their significance to Tamala's journey. The characters' relationships evolve as they confront their own fears and desires, adding emotional depth to the narrative. The story culminates in a climactic confrontation that challenges Tamala to make a pivotal choice, one that will determine the fate of the world and her own existence.