"Long Riders!" is a 12-episode anime series that centers on the experiences of Aoi Amuro, a university student who becomes fascinated with cycling after encountering a folding bike. Intrigued by the idea of owning her own bicycle, Aoi purchases a used folding bike named "Paipai" and begins her journey into the world of cycling. As she learns the basics of bike maintenance, road safety, and the joys of riding, Aoi discovers a community of fellow cycling enthusiasts, including her classmates and friends.

The narrative explores Aoi's growth as a cyclist, from her initial struggles with balancing and endurance to her participation in group rides and cycling events. Alongside Aoi, the series introduces a diverse cast of characters, each with their own unique relationship to cycling. These include Ami Kurata, Aoi's energetic and supportive friend who shares her passion for cycling; Hinako Saijō, a skilled and experienced cyclist who becomes a mentor figure; and Yayoi Ichinose, a reserved but determined rider who gradually opens up through her interactions with the group.

The series emphasizes the camaraderie and challenges of cycling, portraying both the physical demands of the sport and the personal connections formed through shared experiences. Aoi and her friends navigate various cycling-related activities, such as long-distance rides, bike maintenance workshops, and participation in cycling clubs. The story also touches on themes of perseverance, self-discovery, and the importance of community, as the characters support one another in overcoming obstacles and achieving their goals.

"Long Riders!" balances lighthearted moments with realistic depictions of cycling culture, offering an accessible and engaging portrayal of the sport. The series provides a detailed look at the technical aspects of cycling, from gear selection to route planning, while also highlighting the emotional and social aspects of the hobby. Through Aoi's journey, the anime captures the sense of freedom and accomplishment that comes with cycling, making it a relatable and inspiring story for both cycling enthusiasts and newcomers alike.