Tsubone serves as an office worker in the accounting department of Carrier Man Trading Co., Ltd. She is a Komodo dragon with grayish-green scales, yellow eyes, a hunched posture, and a constantly protruding forked tongue. Her physical traits include raised forehead bumps and bright red lipstick, typically dressed in a navy blue vest and skirt over a white dress shirt, brown shoes, and glasses with a chain.

Her condescending and sexist nature manifests through passive-aggressive tactics. She disproportionately burdens female colleagues like Retsuko with her workload while blushing and accommodating male coworkers. She refuses assistance from male subordinates such as Haida for trivial tasks like opening jars. Masking her intentions with false sweetness, she feigns compliments before overloading Retsuko with extra duties. Her sarcasm, unfairness, and volatile anger escalate to throwing objects in the office, fostering a hostile environment where she brands Retsuko as lazy or incompetent.

Tsubone shares a long professional history with Director Ton, dating back to the accounting division's early days. They reminisce about past indulgences with company funds. Demonstrating loyalty, she becomes the sole visitor to Ton's new office after his demotion in Season 4, hinting at a deeper personal bond beneath her antagonism.

Professionally, she resists modernization, reacting defensively when asked to update her tech-illiterate work methods. A recurring habit involves challenging colleagues to open her tightly sealed snack jars to witness their struggles. Her role remains static across the series and media, with no significant development or expanded backstory. Interactions consistently reinforce her workplace antagonism and selective camaraderie.