"Seiyū Radio no Ura Omote" centers on the lives of two high school girls, Yuhi Yugure and Ichinose Chika, who are both voice actresses navigating the complexities of their professional and personal lives. Yuhi is a cheerful and outgoing individual, while Ichinose is more reserved and serious. Despite their contrasting personalities, they are cast as co-hosts for a popular radio show aimed at promoting their latest anime project. The series explores the dynamic between the two as they balance their on-air personas with their off-air realities.

The narrative delves into the challenges of the voice acting industry, highlighting the pressures of maintaining public images, meeting fan expectations, and managing personal insecurities. Yuhi and Ichinose often clash due to their differing approaches to their work and interactions with fans, but their shared experiences and mutual respect gradually foster a deeper understanding and camaraderie. The radio show serves as a backdrop for their evolving relationship, with each episode revealing more about their individual struggles and growth.

Supporting characters include their manager, fellow voice actors, and fans, all of whom contribute to the broader context of the voice acting world. The series also touches on themes of friendship, identity, and the pursuit of dreams, offering a nuanced portrayal of the entertainment industry. Through its focus on the behind-the-scenes aspects of voice acting, "Seiyū Radio no Ura Omote" provides an insightful look into the lives of those who bring animated characters to life.