"Sparrow's Hotel" is a short-form anime series set in a fictional hotel of the same name. The narrative revolves around the daily interactions and comedic misadventures of the hotel staff, particularly focusing on the new employee, Kaoruko Hanayashiki. Kaoruko, a former assassin with a mysterious past, joins the hotel as a front desk clerk, attempting to leave her violent history behind and adapt to a normal life. Her unique skill set often clashes with the mundane tasks of hotel work, leading to humorous and absurd situations.

The hotel's manager, Mr. Suzuki, is a stern but well-meaning figure who remains largely unaware of Kaoruko's background. His interactions with her and the rest of the staff highlight the contrast between her lethal capabilities and the trivialities of customer service. Other characters include the hotel's chef, who occasionally becomes entangled in Kaoruko's antics, and various guests whose quirks and demands provide additional comedic fodder.

The series is episodic, with each short episode presenting a self-contained scenario that explores the absurdity of blending Kaoruko's deadly expertise with the hospitality industry. The humor often stems from her over-the-top reactions to mundane problems, such as dealing with difficult guests or handling minor workplace conflicts. Despite her attempts to fit in, her instincts as an assassin frequently surface, creating a recurring theme of juxtaposition between her past and present life.

The tone is lighthearted and comedic, with a focus on situational humor rather than deep character development or overarching plotlines. The brevity of the episodes and the straightforward premise make the series a quick, entertaining watch, emphasizing the contrast between Kaoruko's lethal background and the ordinary world of hotel management.