"Pittanko! Nekozakana" is a short-form anime series that revolves around the daily lives of anthropomorphic cats who work at a small fishing company called Nekozakana. The company specializes in catching and selling fish, but the employees, all of whom are cats, often find themselves in humorous and absurd situations as they navigate their work and personal lives. The series is episodic, with each episode focusing on a different comedic scenario or challenge faced by the characters.

The main characters include the diligent and serious-minded Boss, who is the head of Nekozakana and often tries to maintain order despite the chaos around him. There is also the laid-back and carefree Tama, who frequently shirks responsibilities but occasionally shows surprising competence. Another key character is the energetic and mischievous Kuro, who often stirs up trouble but has a good heart. The team is rounded out by the shy and reserved Shiro, who tends to be overlooked but occasionally surprises everyone with her hidden talents.

The plotlines are lighthearted and often revolve around the cats' attempts to catch fish, manage their business, or deal with interpersonal dynamics. For example, one episode might involve the cats trying to outsmart a particularly elusive fish, while another might focus on their efforts to host a successful company event. The humor is derived from the cats' exaggerated personalities, their interactions with each other, and the absurdity of their situations. Despite their anthropomorphic nature, the cats face relatable workplace challenges, making the series both whimsical and grounded in its humor.

The series is characterized by its fast-paced, comedic tone and its use of visual gags and slapstick humor. The animation style is simple and playful, reflecting the light-hearted nature of the story. While the series does not delve deeply into character development or complex narratives, it succeeds in delivering consistent entertainment through its quirky premise and charming cast of characters.