Ayumi Yoshida is a first-grade student at Teitan Elementary School and a founding member of the Detective Boys. This group formed after investigating a supposedly haunted mansion that hid an unsolved homicide case, an investigation she shared with Conan Edogawa, Genta Kojima, and Mitsuhiko Tsuburaya, leading to their ongoing collaboration. Ayumi remained the group's sole female member until Ai Haibara joined. Her accidental discovery of a treasure map later prompted the group to hunt for stolen Italian gold coins; Conan saved them from pursuing mafia members during this hunt, an event that sparked Ayumi's serious romantic crush on Conan.
Ayumi displays a primarily naive and innocent personality, often showing courage during investigations and acting as the group's cheerful spirit. She openly expresses her romantic interest in Conan, which causes him discomfort and fuels jealousy in Mitsuhiko and Genta, both of whom harbor crushes on her that she fails to notice. Ayumi occasionally interprets Conan's protective actions toward Haibara as romantic feelings between them. Despite this, she gradually forms a closer friendship with Haibara, who allows Ayumi exclusive use of the informal "Ai-chan." During cases, Ayumi contributes through simple yet perceptive observations, like deciphering a code from a Momotaro container by linking it to statue positioning at the Oohara Art Museum, requiring 25 steps in the direction a statue faced.
She lives with her parents as their only child. Ayumi owns a pink cellphone featuring a wallpaper image of herself playing with a sparkler. Her name carries Japanese meanings: "Ayumi" translates to "beautiful step," while "Yoshida" signifies "joyful ricefield." Physically, she has short brown hair typically worn with a hairband, blue eyes, and frequently wears dresses.
In *Detective Conan: The Scarlet Bullet*, Ayumi appears with the Detective Boys during events surrounding the Tokyo World Sports Games (WSG). She participates in the investigation of kidnappings targeting figures linked to the games, specifically helping locate the kidnapped Shiro Suzuki at a WSG pre-opening party venue. She participates without significant personal development or distinct storylines specific to her within the film.