"Otoboku - Maidens Are Falling for Me!" centers on Mizuho Miyanokouji, a young man who is forced to attend an all-girls school due to a family tradition. To comply with the school's strict female-only policy, Mizuho disguises himself as a girl, adopting the appearance and mannerisms expected of a female student. The story unfolds at the prestigious all-girls academy, where Mizuho becomes the object of affection for several of his classmates, creating a series of comedic and romantic situations.

Mizuho's enrollment is facilitated by his cousin, Mariya Mikado, who is aware of his true identity and serves as a confidante throughout the series. Mariya is a prominent figure at the school and plays a key role in helping Mizuho navigate the challenges of maintaining his disguise. As Mizuho settles into school life, he attracts the attention of multiple girls, including the cheerful and energetic Kana Nakatsukasa, the reserved and studious Shion Sumeragi, and the mischievous and playful Akane Aoi. Each of these characters develops feelings for Mizuho, leading to a blend of lighthearted humor and emotional moments as they grapple with their affections.

The narrative explores themes of identity, gender roles, and the complexities of relationships within the unique setting of an all-girls school. Mizuho's dual identity creates tension as he struggles to balance his true self with the expectations placed upon him, while also managing the romantic advances of his peers. The series combines elements of romantic comedy with occasional dramatic undertones, as the characters confront their feelings and the challenges of their circumstances.

Throughout the story, Mizuho's relationships with the other characters evolve, with each girl bringing her own personality and perspective to the dynamic. The interactions are driven by a mix of misunderstandings, heartfelt confessions, and comedic mishaps, all set against the backdrop of school life. The series maintains a focus on the romantic entanglements and personal growth of the characters, culminating in a resolution that addresses the central conflicts and relationships.