"Tsurune: Kazemai Kōkō Kyūdō-bu" centers on Minato Narumiya, a high school student who rediscovers his passion for kyūdō, the traditional Japanese martial art of archery, after a traumatic incident caused him to abandon it. Minato joins the kyūdō club at Kazemai High School, where he reconnects with his childhood friends Seiya Takehaya and Ryohei Yamanouchi, both of whom are also members of the club. The narrative explores Minato's journey as he overcomes his psychological struggles, particularly his "target panic," a condition that causes him to freeze when aiming at the target.

The series delves into the dynamics of the kyūdō club, highlighting the camaraderie and rivalries among its members. Alongside Minato, Seiya, and Ryohei, the club includes Nanao Kisaragi, a cheerful and skilled archer, and Kaito Onogi, a reserved but dedicated member. The group is guided by their coach, Masaki Takigawa, a former professional kyūdō practitioner who provides mentorship and challenges the team to grow both individually and collectively.

As the story progresses, the Kazemai kyūdō club prepares for competitions, facing off against rival schools such as Kirisaki High School, led by the talented and enigmatic Shū Fujiwara. These competitions serve as a backdrop for character development, as each member confronts their personal insecurities and strives to improve their skills. The series emphasizes the philosophical and spiritual aspects of kyūdō, portraying it not just as a sport but as a means of self-discovery and emotional healing.

The narrative also explores Minato's relationships with his family, particularly his younger sister, Yuna, and his late mother, whose influence on his love for kyūdō is revealed through flashbacks. These elements add depth to Minato's character and provide context for his emotional journey. Through its focus on teamwork, perseverance, and the pursuit of personal growth, "Tsurune: Kazemai Kōkō Kyūdō-bu" offers a heartfelt exploration of kyūdō and its impact on the lives of its practitioners.