"Ah My Buddha" centers around Ikkou Satonaka, a young man raised in a Buddhist temple who possesses a unique ability: he can see and communicate with spirits. This ability often leads to chaotic situations, as Ikkou is easily influenced by the supernatural entities around him. Despite his upbringing, Ikkou struggles with maintaining a disciplined, monk-like demeanor, frequently succumbing to his more impulsive and lustful tendencies. His life takes a dramatic turn when he enrolls at Ryugenzan Temple, a training ground for young monks and priestesses, where he meets a group of female trainees, each with their own quirks and spiritual abilities.

The series blends comedy, romance, and supernatural elements, with much of the humor arising from Ikkou's interactions with the female characters and his inability to control his reactions to their charms. Among the main characters is Chitose Nanjou, a skilled and serious priestess-in-training who often clashes with Ikkou due to his lack of discipline. Other notable characters include Kaede Sakura, a cheerful and energetic girl with a strong connection to nature spirits, and Botan, a shy and reserved girl who harbors feelings for Ikkou but struggles to express them. The dynamics between Ikkou and the female trainees are further complicated by the presence of various spirits, demons, and supernatural phenomena that frequently disrupt their daily lives.

As the story progresses, Ikkou and his companions face a series of challenges that test their spiritual abilities and personal growth. These challenges often involve exorcising malevolent spirits, resolving conflicts between humans and supernatural beings, and uncovering the mysteries surrounding their own powers. The narrative occasionally delves into more serious themes, such as the consequences of misusing spiritual abilities and the importance of self-control, but these are typically balanced by lighthearted and comedic moments. The series maintains a focus on the interplay between Ikkou's flawed yet well-meaning personality and the diverse cast of characters, creating a blend of episodic adventures and overarching plotlines that explore the complexities of their relationships and spiritual journeys.