"Yuri Is My Job!" centers around Hime Shiraki, a high school student who prides herself on maintaining a perfect, princess-like demeanor to gain admiration from others. Her carefully constructed image is disrupted when she accidentally injures Mitsuki Yano, a café worker, during a confrontation. To avoid a lawsuit, Hime agrees to take Mitsuki’s place at Café Liebe, a themed café where staff members roleplay as students at an all-girls boarding school, complete with romanticized relationships between the "students."

At Café Liebe, Hime is paired with Kanoko Mitsuya, a reserved and seemingly indifferent coworker who harbors a deep admiration for Hime. Kanoko’s quiet demeanor masks her intense feelings, creating a dynamic that oscillates between tension and camaraderie. As Hime navigates her new role, she struggles to reconcile her performative personality with the genuine emotions she begins to experience, particularly in her interactions with Kanoko and the other staff members.

The café setting serves as a backdrop for exploring themes of identity, performance, and the complexities of human relationships. The staff members, including the cheerful Sumika Chikura and the enigmatic Mai Koshiba, each bring their own personalities and struggles to the workplace, adding layers to the interpersonal dynamics. The narrative delves into the contrast between the idealized relationships portrayed in the café and the real-life challenges the characters face, highlighting the gap between appearance and reality.

As the story progresses, Hime’s relationships with her coworkers evolve, particularly her bond with Kanoko, which becomes a focal point of emotional development. The series examines how the characters navigate their roles within the café while confronting their personal insecurities and desires. Through its blend of lighthearted moments and introspective themes, "Yuri Is My Job!" offers a nuanced portrayal of its characters and their journeys within the unique environment of Café Liebe.