"CUE!" is an anime series centered around the lives of aspiring voice actors working at the fictional talent agency AiRBLUE. The narrative explores their journey as they navigate the challenges of the voice acting industry, balancing personal growth, professional development, and interpersonal relationships. The story is structured around the agency's 16 young talents, each with distinct personalities, backgrounds, and aspirations, as they strive to make a name for themselves in a competitive field.

The series begins with the introduction of Haruna Moriya, a reserved and inexperienced newcomer who joins AiRBLUE as a voice actor trainee. Her journey serves as a focal point, highlighting the struggles and triumphs of breaking into the industry. Alongside Haruna, the other members of AiRBLUE, including the confident and ambitious Yuna Hina, the cheerful and supportive Rio Kazama, and the diligent and serious Ayana Tsubaki, work together to improve their skills and secure roles in various projects. Each character faces unique challenges, such as overcoming self-doubt, managing rivalries, and adapting to the demands of their craft.

The plot unfolds through a series of episodic arcs, focusing on individual characters and their personal growth while also emphasizing teamwork and camaraderie. The voice actors participate in auditions, recording sessions, and training exercises, providing insight into the technical and emotional aspects of the profession. The series also delves into the relationships between the characters, exploring friendships, rivalries, and mentor-student dynamics. As the story progresses, the characters gradually mature, gaining confidence and honing their abilities, while also learning to support one another in their shared pursuit of success.

The narrative balances lighthearted moments with more serious themes, such as the pressure to succeed, the fear of failure, and the importance of perseverance. Through its ensemble cast, "CUE!" offers a multifaceted portrayal of the voice acting industry, highlighting both its challenges and rewards. The series concludes with the characters reflecting on their growth and looking ahead to the future, leaving their ultimate success open-ended but emphasizing the value of their journey and the bonds they have formed.