Jessie’s role in *Pokémon: The Rise of Darkrai* aligns with her recurring Team Rocket antics, featuring brief but spirited appearances. Alongside James and Meowth, she launches a scheme to snatch Drifloon and Drifblim aboard a Carnivine-shaped hot air balloon, only to be foiled when the Pokémon retaliate with Gust, catapulting the trio skyward. Later, they surveil Darkrai from afar, with Jessie praising its Dark Void technique for its tactical potency.

Her broader backstory, established throughout the anime, traces a life shaped by childhood hardships, including poverty and dashed dreams—failed attempts at Pokémon nursing school and idol pursuits. Her mother, Miyamoto, a decorated Team Rocket operative, disappeared during a covert operation, leaving Jessie in foster care. She later channeled her flair for theatrics into Pokémon contests, adopting aliases like "Jessilina" to compete, though victories remained fleeting.

Jessie’s personality blends sharp-tongued vanity, fiery impatience, and unexpected moments of compassion, particularly toward serpentine or Poison-type Pokémon like Seviper. Despite perpetual setbacks in her quest to seize Ash’s Pikachu, she clings to relentless ambition, sparring with rival agent Cassidy and asserting authority within her trio through bold, if ill-fated, leadership.