Jessie serves as a member of Team Rocket during the Alto Mare incident, collaborating with James and Meowth to surveil and disrupt local operations. The trio uncovers Annie and Oakley as rogue agents within their organization and covertly tracks their activities, seeking to hijack their plot involving the Soul Dew and claim credit for its defeat. Preferring subterfuge over direct clashes, they employ eavesdropping, sabotage, and misdirection to hinder both the antagonists and protagonists from afar.

Jessie’s behavior adheres to Team Rocket’s broader goals of seizing rare Pokémon and artifacts, though her personal motives remain unexplored. Her engagement centers on tactical coordination with her teammates and reactive opposition to Annie and Oakley, avoiding meaningful contact with primary heroes. The narrative refrains from elaborating on her history, external missions, or evolution beyond this event, maintaining focus on her role within the Alto Mare conflict.