"Wake Up, Girls!: The Movie" serves as a prequel to the "Wake Up, Girls!" anime series, providing backstory and context to the formation of the idol group and their struggles. The narrative centers on Green Leaves Entertainment, a struggling talent agency in Sendai, Japan, and its manager, Tange. Facing financial ruin and the departure of their only successful idol, Tange decides to form a new idol group to save the agency. The story introduces seven girls, each with their own personal challenges and aspirations, who come together to form the group "Wake Up, Girls!"

The film explores the individual lives of the girls before they become idols. Mayu, the youngest, struggles with her self-esteem and family expectations. Airi, a former child actress, faces pressure from her overbearing mother. Miyu, a reserved and introspective girl, grapples with her lack of confidence. Nanami, a cheerful and optimistic character, works part-time jobs to support her family. Kaya, a former delinquent, seeks redemption through her passion for dance. Minami, a diligent and responsible girl, balances her studies with her dreams of performing. Finally, Yoshino, a former member of a rival idol group, joins the team after leaving her previous agency due to creative differences.

As the group begins training, they face numerous obstacles, including financial difficulties, internal conflicts, and the harsh realities of the idol industry. The film highlights their determination to overcome these challenges, showcasing their growth as individuals and as a team. The narrative culminates in their first live performance, where they must prove their worth to both their audience and themselves. The movie sets the stage for the events of the anime series, emphasizing the themes of perseverance, teamwork, and the pursuit of dreams in a competitive and often unforgiving industry.