"Wake Up, Girls!" is a 12-episode anime series that centers on the struggles and aspirations of a fledgling idol group in Sendai, Japan. The narrative begins with the formation of the group under the guidance of a struggling talent agency, Green Leaves Entertainment, which is on the brink of collapse due to financial difficulties and the departure of its top idol. The agency’s president, Tange, and his assistant, Matsuda, decide to create a new idol group to revive the company’s fortunes. They recruit seven girls with varying backgrounds and levels of experience, each facing personal challenges and uncertainties about their futures.

The main characters include Mayu Shimada, a former child actress seeking a fresh start; Airi Hayashida, a shy and reserved girl with a passion for singing; Miyu Okamoto, a diligent and responsible high school student; Nanami Hisami, a cheerful and energetic girl with a love for dancing; Kaya Kikuma, a former delinquent trying to turn her life around; Minami Katayama, a quiet and introspective girl with a mysterious past; and Yoshino Nanase, a confident and outspoken individual who becomes the group’s de facto leader. Together, they form "Wake Up, Girls!" and begin their journey toward becoming professional idols.

The series explores the harsh realities of the idol industry, including intense competition, grueling training, and the pressure to succeed. The girls face numerous obstacles, such as internal conflicts, self-doubt, and the skepticism of others. Their journey is marked by both triumphs and setbacks, including a disastrous debut performance that forces them to reevaluate their commitment and resolve. As they work to improve their skills and build a fanbase, they also confront personal issues, such as family expectations, past traumas, and the sacrifices required to pursue their dreams.

Throughout the series, the group’s growth is depicted through their performances, interactions with fans, and the bonds they form with one another. The narrative emphasizes the importance of perseverance, teamwork, and self-belief in overcoming challenges. By the end of the series, "Wake Up, Girls!" begins to gain recognition, but their journey is far from over, leaving room for further development and exploration of their careers and relationships. The series provides a grounded and realistic portrayal of the idol industry, balancing moments of humor, drama, and heartfelt emotion.