"Go! Princess Precure" is a magical girl anime that centers around Haruka Haruno, a cheerful and determined middle school student who dreams of becoming a princess. Her life takes a dramatic turn when she encounters a fairy named Pafu, who reveals that Haruka has been chosen to become a Precure warrior. Alongside two other girls, Minami Kaido and Kirara Amanogawa, Haruka transforms into Cure Flora, Cure Mermaid, and Cure Twinkle, respectively. Together, they are tasked with protecting the Hope Kingdom, a magical realm, from the forces of despair led by Dyspear and her minions, who seek to plunge the world into eternal darkness by stealing the "Dress Up Keys," magical items tied to dreams and aspirations.

The narrative unfolds as the trio balances their everyday lives with their duties as Precure warriors. Haruka, despite her initial clumsiness and lack of confidence, grows into her role as a leader, embodying the ideals of courage and perseverance. Minami, a graceful and intelligent girl, struggles with self-doubt but finds strength in her friendships. Kirara, a passionate and ambitious idol, learns to reconcile her personal dreams with her responsibilities as a Precure. The series emphasizes themes of hope, dreams, and the importance of never giving up, as the girls face increasingly challenging battles against Dyspear's forces, including the Zetsuborgs, creatures born from despair.

As the story progresses, the Precure warriors uncover the truth about Dyspear's origins and her connection to the Hope Kingdom. They also encounter Towa Akagi, a mysterious girl with a tragic past, who becomes a pivotal figure in the battle against despair. Towa's journey from antagonist to ally adds depth to the narrative, highlighting the power of redemption and forgiveness. The climax of the series sees the Precure warriors confronting Dyspear in a final battle to restore hope and dreams to both the Hope Kingdom and the human world. Through their unwavering determination and the bonds they share, they ultimately triumph, reaffirming the series' core message that dreams are worth fighting for.