"Anime-Gataris" centers around Minoa Asagaya, a high school freshman who joins the Anime Research Club (ARC) at Sakaneko Private High School. Initially, Minoa has little knowledge of anime but is drawn to the club after a mysterious upperclassman, Arisu Kamiigusa, encourages her to explore her interest. The club, which is on the verge of being disbanded due to low membership, consists of a small group of students with varying levels of anime enthusiasm. These include the passionate and knowledgeable club president, Kouki Mizunashi, the quiet and reserved Maon Sakaki, the energetic and otaku-culture-loving Erika Ishigaki, and the stoic yet supportive Miko Kouzuki.

As the story progresses, the club members bond over their shared love for anime, discussing various genres, tropes, and industry trends. The narrative blends slice-of-life elements with comedic moments, often parodying well-known anime conventions and clichés. The characters frequently engage in meta-commentary about the anime industry, creating a self-aware tone that appeals to seasoned anime fans.

The plot takes an unexpected turn when the club discovers a mysterious AI named "Animus" within their school’s computer system. Animus begins to manifest strange phenomena, blurring the line between reality and the anime world. This development introduces a surreal and fantastical layer to the story, as the characters must navigate increasingly bizarre situations that challenge their understanding of their own reality. The series balances its comedic and lighthearted tone with moments of introspection, particularly as the characters confront their personal struggles and aspirations.

Throughout the series, the ARC members work together to save their club from disbandment while unraveling the mystery of Animus. The narrative explores themes of friendship, creativity, and the transformative power of shared passions. By the end, the characters grow closer, not only as anime enthusiasts but as individuals who find strength in their collective experiences. "Anime-Gataris" offers a unique blend of humor, meta-narrative, and heartfelt moments, making it a distinctive entry in the anime genre.