"Spider Riders" is a 52-episode anime series based on the toyline and book series of the same name. The story is set in a subterranean world called the Inner World, where humans coexist with intelligent, battle-ready spiders. The Inner World is under constant threat from the Invectids, a race of insect-like creatures led by the malevolent Mantid. To defend their world, humans form alliances with spiders, becoming Spider Riders who wield powerful Arachna Armor and fight alongside their spider partners.

The protagonist, Hunter Steele, is a young boy from the Surface World who accidentally stumbles into the Inner World through a portal. Upon arrival, he bonds with a spider named Shadow, becoming a Spider Rider. Hunter discovers he is the Oracle's chosen one, destined to wield the Oracle's power to protect the Inner World. Alongside other Spider Riders—Corona, a skilled and determined warrior, and Igneous, a hot-headed but loyal fighter—Hunter trains and battles the Invectids. The group is guided by Lumen, a wise and enigmatic figure who serves as their mentor.

The narrative revolves around the Spider Riders' efforts to prevent Mantid and his forces from obtaining the Oracle's power, which would grant them control over both the Inner and Surface Worlds. The series explores themes of teamwork, courage, and self-discovery as Hunter grows into his role as a leader. Key plot points include the discovery of ancient ruins, the unlocking of new Arachna Armor abilities, and the revelation of deeper connections between the Inner World and the Surface World. The story also delves into the origins of the Invectids and their conflict with the humans and spiders, adding layers of complexity to the ongoing war.

Throughout the series, the Spider Riders face numerous challenges, including internal conflicts, betrayals, and the constant threat of Mantid's schemes. The dynamic between the characters evolves as they learn to trust one another and harness their unique strengths. The series balances action-packed battles with moments of character development, creating a cohesive narrative that appeals to a younger audience while maintaining a sense of adventure and intrigue.