"Prince of Stride: Alternative" is a sports anime centered around the fictional extreme sport of "Stride," a high-energy relay race combining parkour, free-running, and sprinting. The narrative unfolds at Honan Academy, where first-year student Nana Sakurai transfers and becomes fascinated by the school's Stride club. Despite its dwindling membership, the club is revitalized when Nana recruits Takeru Fujiwara, a talented but reluctant runner, and Riku Yagami, a transfer student with a natural aptitude for the sport. Together, they aim to restore the club's former glory and compete in the End of Summer tournament.

The series explores the dynamics of teamwork and personal growth as the Honan Stride team trains and competes against rival schools. Key characters include team captain Heath Hasekura, whose calm demeanor and strategic mind guide the group; Ayumu Kadowaki, a cheerful and supportive member; and Hozumi Kohinata, a skilled runner with a playful personality. The team's interactions highlight their individual struggles and motivations, such as Takeru's initial reluctance to join due to past trauma and Riku's determination to prove himself.

As the story progresses, the team faces various challenges, both on and off the track. They encounter formidable opponents, including the elite Stride teams from other schools, each with unique running styles and strategies. The narrative emphasizes the importance of trust, communication, and perseverance, as the Honan team learns to synchronize their movements and emotions to achieve peak performance. The End of Summer tournament serves as the climax, testing their skills and resolve while showcasing the exhilarating and fast-paced nature of Stride.

The series balances sports action with character-driven moments, delving into the personal lives and relationships of the team members. Themes of friendship, ambition, and overcoming adversity are woven throughout the narrative, creating a compelling blend of athletic competition and emotional depth. "Prince of Stride: Alternative" offers a vibrant and engaging portrayal of a unique sport, capturing the excitement of teamwork and the pursuit of excellence.