"Mahō Sensei Negima!: Mō Hitotsu no Sekai" is a four-episode OVA series that serves as an alternate retelling of the "Mahō Sensei Negima!" manga, diverging from the original anime adaptation. The story centers on Negi Springfield, a ten-year-old prodigy wizard from Wales, who is sent to Japan to teach English at Mahora Academy as part of his training to become a master mage. Negi’s class, 2-A, consists of 31 unique female students, each with distinct personalities and backgrounds. Early in the series, Negi’s magical identity is discovered by Asuna Kagurazaka, a spirited and protective classmate, who becomes his first pactio partner—a magical contract that grants her enhanced abilities in exchange for supporting him.

The narrative takes a significant turn when Negi and his students are transported to a parallel world known as the "Mundus Magicus," a magical realm where technology and magic coexist. This world is under threat from a mysterious organization seeking to exploit its resources and destabilize its balance. Negi and his students must navigate this unfamiliar environment, encountering allies and adversaries while uncovering the truth behind the organization’s motives. Key characters such as Evangeline A.K. McDowell, a powerful vampire and former antagonist, and Fate Averruncus, a enigmatic and formidable mage, play pivotal roles in the unfolding conflict.

As the story progresses, Negi’s relationships with his students deepen, and their individual strengths and abilities become crucial in overcoming challenges. The series explores themes of camaraderie, responsibility, and the clash between magical and technological forces. The OVA condenses and reimagines key arcs from the manga, focusing on high-stakes battles, character development, and the intricate world-building of the Mundus Magicus. While the OVA provides a self-contained narrative, it retains the core elements of the original story, offering a fresh perspective for both new and returning viewers.